Journal: Stem Cell Research & Therapy
Article Title: Overexpression of SOX4 in MSCs inhibits cellular senescence and enhances therapeutic efficacy in systemic lupus erythematosus
doi: 10.1186/s13287-025-04525-w
Figure Lengend Snippet: Overexpression of SOX4 in hUC-MSCs enhances their therapeutic efficacy in systemic lupus erythematosus. A , B IMQ induced lupus mice were treated with PBS, P3-MSCs or P8-MSCs. CD4 + PD-1 + and CD4 + ICOS + T cells in spleen were detected using FCM. C-J Imiquimod (IMQ) induced lupus mice were treated with PBS, control (pc-ctrl) or SOX4 overexpressed P8-MSCs (pc-sox4). D Representative images of spleens and cervical draining lymph node from mice. E , F Body weight, the ratio of spleen or kidney weight to body weight, number of cells in spleen and lymph node of mice. G Serum ANA was detected using ELISA. H H&E, MASSON or PAS staining of kidney. I , J CD4 + PD-1 + and CD4 + ICOS + T cells in spleen were detected using FCM. n = 3 or 4, * p < 0.05, ** p < 0.01, *** p < 0.001. Symbols represent individual subjects
Article Snippet: Serum levels of anti-nuclear antibodies (ANA) were quantified using Mouse ANA ELISA Kit (Shibayagi, Gunma, Japan), following the manufacturer’s protocol.
Techniques: Over Expression, Drug discovery, Control, Enzyme-linked Immunosorbent Assay, Staining